27NOV-07DEC AKTA for IRM12, in vitro protein synthesis
27NOV17 I prepared 1lt of water, 1/2 lt of buffer A, 1/2 lt buffer B for IRM12 and 1/2 lt of Ethanol 20%. I need to degass them before AKTA. I took a fennel (storage room at the back in a plastic box), a cylinder 1lt and 4 stirring stones. I put the water into the cylinder. Place the stirring stone in the empty water bottle and place it on the stirring plate. I adjusted the fennel onto the bottle and strewed it and then I connected the degassing tube to the fennel. I turn the degassing machine on and pour some of the water very gently into the fennel. I want the filter to be soaked first and then I put the rest of the water. I place the lid and turn the stirring plate on. The degassing has been finished once there no bubbles on the stirring stone. The degased buffers can be kept for a day degased so I can run the AKTA tomorrow morning 28NOV17 I run the AKTA using the degased buffers for purifying the IRM12 and collected all the 30 fractions produced. I also saved the s...