22NOV17 in vitro protein synthesis IRM6-13 positive and negative kit controls

22NOV17

Tape Station for IRM1 -13, B99 and kit positive, negative included

I followed the instructions of the Tape Station. I prepared the samples exactly as it says in the Tapestation instructions.

I added a tape with the barcode outside and down (the program needs to recognise it). I open the tape station controller software 2200. I place the tubes without the lids in the places at the left, I also place some tips at the right side (using the multipipette). The ladder needs to be at position 1. Press start. Save the results and also produce a pdf report.


The bands produced are as follows on page



Qubit concentration calculation

I calculated the concentration of the protein produced in the in vitro protein synthesis kit (in mg/ml)

IRM1: 2.22
IRM2: 2.6
IRM3: 1.43
IRM4: 2.16
IRM5: 2.32
IRM6: 2.52
IRM7: 2.38
IRM8: 2.54
IRM9: 2.58
IRM10: 1.94
IRM11: 2.38
IRM12: 2.4
IRM13: 2.64
B99: 2.3
Kit negative: 2.68
Kit positive: 2.88