ASSAYS TO BE DONE

PNPG PNPX substrate activity assay

aliquots of diluted enzyme

100μl of 1.25mM substrate in

50mM buffer for 5 min at 40oC or 42oC (depending on the enzyme)

Quench the reaction with 100μl 1M Na2CO3

pH optimum

Temperature optima

Thermostability


Specificity of enzymes towards different substrates
PNPG
PNPX
cellobiose
MuC

Influence of metal cations, monosaccharides, and alcohols

Substrate selection of degree of polymerasation


Synergism with xylanase

1:1 to 10:1