IRM12 SONICATION and AKTA PURIFICATION 18DEC-22DEC
18-22DEC17
I made a new IRM12 E.coli culture and sonicate it using the DR 0817-073 page 116 recipe only with 1% glycerol always. I took the lysate and run an AKTA.
The AKTA computer was not starting up so I did it manually using only the machine. The data were not stored and therefore I am not able to see at the details of the AKTA but I did take the fractions out. I froze everything at -20oC to stay over the Christmas period and I will run a gel containing all the fractions for detecting the protein after the company reopen again.
I made a new IRM12 E.coli culture and sonicate it using the DR 0817-073 page 116 recipe only with 1% glycerol always. I took the lysate and run an AKTA.
The AKTA computer was not starting up so I did it manually using only the machine. The data were not stored and therefore I am not able to see at the details of the AKTA but I did take the fractions out. I froze everything at -20oC to stay over the Christmas period and I will run a gel containing all the fractions for detecting the protein after the company reopen again.
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