02Feb18

02FEB18

There is an idea about the NaCl concentration in the dialysis mixture. The NaCl concentration is 500mM which is higher than the inside the bag NaCl concentration which means that NaCl gets into the bag during dialysis.

I need to check this

Also I need to check whether Imidazole gets out from the bag after dialysis. To check than I need to measure the Imidazole concentration at 280nm before dialysis and after.

Also measure the concentration of protein before dialysis and after.

Dropping the ions concentration (might be high)

Repeat the experiment with the ions and no substrate as control